R4260
Sigma
Monoclonal Anti-CD19−Quantum Red¿ antibody produced in mouse
clone SJ25-C1, purified immunoglobulin, buffered aqueous solution
| Synonym: | Monoclonal Anti-CD19 |
| MDL number: | MFCD02184288 |
Description
| Antigen Background | CD19 is a member of the immunoglobulin superfamily and has two Ig-like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of κ or λ light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. |
| Immunogen | NALM-1 human cell line. |
| Preparation Note | Quantum Red is a tandem fluorescent dye created by coupling R-phycoerythrin with Cy5 reactive dye. This red dye is efficiently excited at 488 nm and emits at 670 nm. |
| Specificity | Recognizes the human CD19 (90 kDa) integral membrane glycoprotein. 3rd Workshop: code no. 073 |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
Properties
| antibody form | purified immunoglobulin |
| clone | SJ25-C1 (monoclonal) |
| form | buffered aqueous solution |
| application(s) | flow cytometry: 10 μL using 1 × 106 cells |
| Gene Information | human ... CD19(930) |
| shipped in | wet ice |
| storage temp. | 2-8°C |
Safety
| WGK Germany | 3 |
